BibTex RIS Kaynak Göster

Rhynchites bacchus L. (Coleoptera: Rhynchitidae)’dan İzole Edilen Beauveria bassiana’nın Moleküler Karakterizasyonu ve Patojenitesi

Yıl 2014, Cilt: 3 Sayı: 2, 33 - 47, 03.02.2015
https://doi.org/10.17100/nevbiltek.210929

Öz

Yaprak kıvrım böceği (Rhynchites bacchus L., Coleoptera: Rhynchitidae) hemen hemen bütün Dünya’da elma, erik, kayısı, kiraz ve şeftali (nektarin) gibi pek çok meyvenin en önemli zararlılarından birisidir. Bu zararlı hem taş hem de yumuşak çekirdekli meyvelerle beslenir ve meyve bahçelerinde %80-90’a kadar zarara neden olabilirler. Bu çalışmada, daha etkili ve güvenli biyolojik mücadele etmeni bulmak açısından bu zararlının fungal patojenleri araştırılmıştır. Böcek örnekleri Türkiye’nin Gümüşhane ilinde 2007 yılının Temmuz ayında R. bacchus ile istila edilmiş elma bahçelerinden toplanmıştır. Bir fungal örnek izole edilmiş ve şu anda kullanılan morfolojik ve ITS, EF1-σ kısmi sekansını içeren moleküler teknikler kullanılarak karakterize edilmiştir. Bu tekniklere dayanarak, bu izolat Beauveria bassiana olarak tanımlanmıştır. Ayrıca bu izolatın R. bacchus’un erginleri üzerindeki insektisidal aktivitesi belirlenmiştir. Fungus 1 × 108 mL-1spor konsantrasyonunun uygulanmasından sonra iki hafta sonunda %100 ölüme neden olmuştur ve bu fungusun LT50 değeri 7,5 gün olarak hesaplanmıştır. Bu çalışma R. bacchus’a karşı B. bassiana’nın etkinliğinin belirlenmesine yönelik ilk çalışmadır

Kaynakça

  • David’yan G.E, “Rhynchites bacchus L. - Peach weevil. http://www.agroatlas.ru/en/content/pests/Rhynchites_bacchus”, 2009
  • Özbek H., Guclu S., Hayat R., Yıldırım E., “Pests of fruit, vineyard and some foliage plant (in
  • Turkish)” Atatürk Üniversitesi Yayınları, 156-157s., 1998
  • Gökçe C., Sevim A., Demirbag Z., Demir I., “Isolation, characterization and pathogenicity of bacteria from Rhynchites bacchus (Coleoptera: Rhynchitidae)” Biocontrol Science and Technolohy, 20, 973-982, 2010
  • The Ministry of Agriculture of Turkey., “Strategy Development Presidency, East Black Sea Regional Agriculture Infinitive Plan (in Turkish)” 35s, 2007
  • Chandler D., Davidson G., Pell J.K., Ball B.V., Shaw K., Sunderland K.D., “Fungal biocontrol of acari” Biocontrol Science and Technolohy, 10, 357-384, 2000
  • Goettel M.S., Eilenberg J., Glare T., “Entomopathogenic fungi and their role in regulation of insect populations”, Edited by Gilbert L.I., Iatrou K., Gill S.S., Comprehensive Molecular Insect Science, Elseiver, Amsterdam, 361-405, 2005
  • Fuxa J.R, “Ecological considerations for the use of Entomopathogens in IPM” Annual Review of Entomology, 32, 225-251, 1987
  • St Leger R., Roberts D.W., “Engineering improved mycoinsecticides” Trends Biotechnology, 15, 83-86, 1997
  • Sevim A., Donzelli B.G.G., Wu D., Demirbag Z., Gibson D.M., Turgeon B.G., “Hydrophobin genes of the entomopathogenic fungus, Metarhizium brunneum, are differentially expressed and corresponding mutants are decreased in virulence” Current Genetics 58, 79-92, 2012
  • Meyling N.V., Eilenberg J., “Ecology of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae in temperate agroecosystems: Potential for conservation biological control” Biological Control, 43, 145-155, 2007
  • Campbell R.R., Anderson T.E., Semel M., Roberts D.W., “Management of the Colorado potato beetle using the entomogenous fungus Beauveria bassiana” American Potato Journal, 61, 29- 37, 1985
  • Leathers T.D., Gupta S.C., “Susceptibility of the eastern tent caterpillar (Malacosoma americanum) to the entomogenous fungus Beauveria bassiana” Journal of Invertebrate Patholohy, 61, 217-219, 1993
  • Padmaja V, Kaur G., “Use of the fungus Beauveria bassiana (Bals.) Vuill (Moniliales: Deuteromycetes) for controlling termites” Current Science, 81, 645-647, 2001
  • Tafoya F., Zuñiga-Delgadillo M., Alatorre R., Cibrian-Tovar J., Stanley D., “Pathogenicity of Beauveria Bassiana (Deuteromycota: Hyphomycetes) against the cactus weevil, Metamasius Spinolae (Coleoptera: Curculionidae) under laboratory conditions” Florida Entomologist, 87, 533-536, 2004
  • Todorova S.I., Cloutier C., Côté J.C., Coderre D., “Pathogenicity of six isolates of Beauveria bassiana (Balsamo) Vuillemin (Deuteromycotina, Hyphomycetes) to Perillus bioculatus (F) (Hem., Pentatomidae)” Journal of Applied Entomology, 126, 182-185, 2002
  • Sevim A., Demir I., Demirbag Z., “Molecular characterization and virulence of Beauveria spp. from the pine processionary moth, Thaumetopoea pityocampa (Lepidoptera: Thaumetopoeidae)” Mycopathologia, 170, 269-277, 2010
  • Sevim A., Demir I., Tanyeli E., Demirbag Z., “Screening of entomopathogenic fungi against the european spruce bark beetle, Dendroctonus micans (Coleoptera: Scolytidae)” Biocontrol Science and Technology, 20, 3-11, 2010
  • Sevim A., Demir I., Hofte M., Humber R.A., Demirbag Z., “Isolation and characterization of entomopathogenic fungi from hazelnut-growing region of Turkey” Biocontrol, 55, 279-297, 2010
  • Sevim A., D., Kocacevik S., Demirbag Z., “Evaluation of entomopathogenic fungi against the sycamore lace bug, Corythucha ciliata (Say) (Hemiptera: Tingidae)” Turkish Journal of Agriculture and Forestry, 37, 595-603, 2013
  • Tanyeli E., Sevim A., Demirbag Z., Eroglu M., Demir I., “Isolation and virulence of entomopathogenic fungi against the great spruce bark beetle, Dendroctonus micans (Kugelann) (Coleoptera: Scolytidae)” Biocontrol Science and Technology, 20, 695-701, 2010
  • Humber R, “Entomopathogenic fungal identification” Edited by Lacey L.A., Manual of Techniques in Insect Pathology, Academic Press, San Diego, 153-185, 1997
  • White T.J., Bruns T., Lee S., Taylor J.W., “AmpliŞcation and direct sequencing of fungal
  • ribosomal RNA genes for phylogenetics” Edited by Innis M.A., Gelfand D.H., Sninsky J.J., White T.J., PCR protocols: A guide to methods and applications, Academic Press, New York, 315-322, 1990
  • Rehner S.A., Buckley E.A., “Beauveria phylogeny inferredfrom nuclear ITS and EF1-α sequences: evidence forcryptic diversification and links to Cordyceps teleomorphs” Mycologia, 97, 84-98, 2005
  • Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J., “Basic local alignment search tool” Journal of Molecular Biology, 215, 403-410, 1995
  • Rehner S.A., Minnis A.M., Sung G.H., Luangsa-ard J.J., Devotto L., Humber R.A., “Phylogeny and systematics of the anamorphic, entomopathogenic genus Beauveria” Mycologia, 103, 1055- 1073, 2001
  • Hall T.A, “BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT” Nucleic Acids Symposium, 41, 95-98, 1999
  • Kimura M., “A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences” Journal of Molecular Evolution, 16, 111-120, 1980
  • Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S., “MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods” Molecular Biology Evolution, 28, 2731-2739, 2011
  • Abbott W.S, “A method of computing the effectiveness of an insecticide” Journal of Economic Entomology, 18, 265-267, 1925
  • Alves S.B., Pereira R.M., Lopes R.B., Tamai M.A., “Use of entomopathogenic fungi in Latin America” Edited by Upadhyay R.K., Advances in Microbial Control of Insect Pests, Kluwer, New York, 193-211s, 2003
  • Feng M.G., Poprawski T.J., Khachatourians G.G., “Production, formulation and application of the entomopathogenic fungus Beauveria bassiana for insect control: current status” Biocontrol Science and Technology, 4, 3-34, 1994
  • Monzon A.J., Guharay F., Klingen I., “Natural occurrence of Beauveria bassiana in Hypothenemus hampei (Coleoptera: Curculionidae) populations in unsprayed coffee fields” Journal of Invertebrate Pathology, 97, 134-141, 2008
  • Chikwenhere G.P.., “Potential effects of Beauveria bassiana (Balsmo) Vuillemin on Neochetina bruchi Hustache (Coleoptera: Curculionidae), a biological control agent of water hyacinth” Biological Control, 21, 105-110, 2001
  • Gulsar Banu J., Subahasan K., Iyer R., “Occurrence and distribution of entomopathogenic nematodes in white grub endemic areas of Kerala” Journal of Plant Crops, 32, 333-334, 2004
  • Bidochka M.J., Menzies F.V., Kamp A.M., “Genetic groups of the insect-pathogenic fungus Beauveria bassiana are associated with habitat and thermal growth preferences” Archives of Microbiology, 178, 531-537, 2002
  • Maurer P., Couteaudier Y., Girard P.A., Bridge P.D., Riba G., “Genetic diversity of Beauveria bassiana and relatedness to host insect range” Mycological Research, 10, 159-164, 1997
  • Sevim A., Hofte M., Demirbag Z., “Genetic variability of Beauveria bassiana and Metarhizium anisopliae var. Anisopliae isolates obtained from the Eastern Black Sea Region of Turkey” Turkish Journal of Biology, 36, 255-265, 2012
  • The Ministry of Agriculture of Turkey, “Struggle with HazelnutPests and Diseases” Turkey, 1992
  • Tafoya F., Zuniga-Delgadillo M., Alatorre R., Cibrian-Tovar J., Stanley D., “Pathogenicity of Beauveria bassiana (Deuteromycota: Hyphomycetes) against the cactus weevil, Metamasius spinolae (Coleoptera: Curculionidae) under laboratory conditions” Florida Entomologist, 87(4), 533-536, 2013
  • Lo Verde G., Torta L., Mondello V., Caldarella C.G., Burruano S., Caleca V., “Pathogenicity bioassays of isolates of Beauveria bassiana on Rhynchophorus ferrugineus. Pest Management Science, doi: 10.1002/ps.3852, 2014

Molecular Characterization and Pathogenicity of Beauveria bassiana Isolated from Rhynchites bacchus L. (Coleoptera: Rhynchitidae)

Yıl 2014, Cilt: 3 Sayı: 2, 33 - 47, 03.02.2015
https://doi.org/10.17100/nevbiltek.210929

Öz

The leafroller weevil beetle (Rhynchites bacchus L., Coleoptera: Rhynchitidae) is one of the most destructive pest of various fruits such as apple, plum, apricot, cherry and peach (nectarine) etc. almost all over the world. This pest feeds either stone or pome fruits and can cause up to 80-90% damage to orchards. In this study, we investigated fungal pathogens of this pest to find more effective and safe biological control agent against it. Insect specimens were collected from apple orchards invaded by R. baccus during July of 2007 in Gümüşhane, Turkey. One fungal species was isolated and characterized by currently used morphological and molecular techniques including ITS and the partial EF1-α sequencing. Based on these characteristics, the isolate was identified as Beauveria bassiana. Separately, the insecticidal activity of the isolate was determined on R. bacchus adults. The fungus caused 100% mortality within two weeks days after application of 1 × 108 ml-1 spore concentration and the LT50 value of the fungus was calculated as 7.5 day. This is the first study to determine the effectiveness of B. bassiana against R. baccus.

Kaynakça

  • David’yan G.E, “Rhynchites bacchus L. - Peach weevil. http://www.agroatlas.ru/en/content/pests/Rhynchites_bacchus”, 2009
  • Özbek H., Guclu S., Hayat R., Yıldırım E., “Pests of fruit, vineyard and some foliage plant (in
  • Turkish)” Atatürk Üniversitesi Yayınları, 156-157s., 1998
  • Gökçe C., Sevim A., Demirbag Z., Demir I., “Isolation, characterization and pathogenicity of bacteria from Rhynchites bacchus (Coleoptera: Rhynchitidae)” Biocontrol Science and Technolohy, 20, 973-982, 2010
  • The Ministry of Agriculture of Turkey., “Strategy Development Presidency, East Black Sea Regional Agriculture Infinitive Plan (in Turkish)” 35s, 2007
  • Chandler D., Davidson G., Pell J.K., Ball B.V., Shaw K., Sunderland K.D., “Fungal biocontrol of acari” Biocontrol Science and Technolohy, 10, 357-384, 2000
  • Goettel M.S., Eilenberg J., Glare T., “Entomopathogenic fungi and their role in regulation of insect populations”, Edited by Gilbert L.I., Iatrou K., Gill S.S., Comprehensive Molecular Insect Science, Elseiver, Amsterdam, 361-405, 2005
  • Fuxa J.R, “Ecological considerations for the use of Entomopathogens in IPM” Annual Review of Entomology, 32, 225-251, 1987
  • St Leger R., Roberts D.W., “Engineering improved mycoinsecticides” Trends Biotechnology, 15, 83-86, 1997
  • Sevim A., Donzelli B.G.G., Wu D., Demirbag Z., Gibson D.M., Turgeon B.G., “Hydrophobin genes of the entomopathogenic fungus, Metarhizium brunneum, are differentially expressed and corresponding mutants are decreased in virulence” Current Genetics 58, 79-92, 2012
  • Meyling N.V., Eilenberg J., “Ecology of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae in temperate agroecosystems: Potential for conservation biological control” Biological Control, 43, 145-155, 2007
  • Campbell R.R., Anderson T.E., Semel M., Roberts D.W., “Management of the Colorado potato beetle using the entomogenous fungus Beauveria bassiana” American Potato Journal, 61, 29- 37, 1985
  • Leathers T.D., Gupta S.C., “Susceptibility of the eastern tent caterpillar (Malacosoma americanum) to the entomogenous fungus Beauveria bassiana” Journal of Invertebrate Patholohy, 61, 217-219, 1993
  • Padmaja V, Kaur G., “Use of the fungus Beauveria bassiana (Bals.) Vuill (Moniliales: Deuteromycetes) for controlling termites” Current Science, 81, 645-647, 2001
  • Tafoya F., Zuñiga-Delgadillo M., Alatorre R., Cibrian-Tovar J., Stanley D., “Pathogenicity of Beauveria Bassiana (Deuteromycota: Hyphomycetes) against the cactus weevil, Metamasius Spinolae (Coleoptera: Curculionidae) under laboratory conditions” Florida Entomologist, 87, 533-536, 2004
  • Todorova S.I., Cloutier C., Côté J.C., Coderre D., “Pathogenicity of six isolates of Beauveria bassiana (Balsamo) Vuillemin (Deuteromycotina, Hyphomycetes) to Perillus bioculatus (F) (Hem., Pentatomidae)” Journal of Applied Entomology, 126, 182-185, 2002
  • Sevim A., Demir I., Demirbag Z., “Molecular characterization and virulence of Beauveria spp. from the pine processionary moth, Thaumetopoea pityocampa (Lepidoptera: Thaumetopoeidae)” Mycopathologia, 170, 269-277, 2010
  • Sevim A., Demir I., Tanyeli E., Demirbag Z., “Screening of entomopathogenic fungi against the european spruce bark beetle, Dendroctonus micans (Coleoptera: Scolytidae)” Biocontrol Science and Technology, 20, 3-11, 2010
  • Sevim A., Demir I., Hofte M., Humber R.A., Demirbag Z., “Isolation and characterization of entomopathogenic fungi from hazelnut-growing region of Turkey” Biocontrol, 55, 279-297, 2010
  • Sevim A., D., Kocacevik S., Demirbag Z., “Evaluation of entomopathogenic fungi against the sycamore lace bug, Corythucha ciliata (Say) (Hemiptera: Tingidae)” Turkish Journal of Agriculture and Forestry, 37, 595-603, 2013
  • Tanyeli E., Sevim A., Demirbag Z., Eroglu M., Demir I., “Isolation and virulence of entomopathogenic fungi against the great spruce bark beetle, Dendroctonus micans (Kugelann) (Coleoptera: Scolytidae)” Biocontrol Science and Technology, 20, 695-701, 2010
  • Humber R, “Entomopathogenic fungal identification” Edited by Lacey L.A., Manual of Techniques in Insect Pathology, Academic Press, San Diego, 153-185, 1997
  • White T.J., Bruns T., Lee S., Taylor J.W., “AmpliŞcation and direct sequencing of fungal
  • ribosomal RNA genes for phylogenetics” Edited by Innis M.A., Gelfand D.H., Sninsky J.J., White T.J., PCR protocols: A guide to methods and applications, Academic Press, New York, 315-322, 1990
  • Rehner S.A., Buckley E.A., “Beauveria phylogeny inferredfrom nuclear ITS and EF1-α sequences: evidence forcryptic diversification and links to Cordyceps teleomorphs” Mycologia, 97, 84-98, 2005
  • Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J., “Basic local alignment search tool” Journal of Molecular Biology, 215, 403-410, 1995
  • Rehner S.A., Minnis A.M., Sung G.H., Luangsa-ard J.J., Devotto L., Humber R.A., “Phylogeny and systematics of the anamorphic, entomopathogenic genus Beauveria” Mycologia, 103, 1055- 1073, 2001
  • Hall T.A, “BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT” Nucleic Acids Symposium, 41, 95-98, 1999
  • Kimura M., “A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences” Journal of Molecular Evolution, 16, 111-120, 1980
  • Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S., “MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods” Molecular Biology Evolution, 28, 2731-2739, 2011
  • Abbott W.S, “A method of computing the effectiveness of an insecticide” Journal of Economic Entomology, 18, 265-267, 1925
  • Alves S.B., Pereira R.M., Lopes R.B., Tamai M.A., “Use of entomopathogenic fungi in Latin America” Edited by Upadhyay R.K., Advances in Microbial Control of Insect Pests, Kluwer, New York, 193-211s, 2003
  • Feng M.G., Poprawski T.J., Khachatourians G.G., “Production, formulation and application of the entomopathogenic fungus Beauveria bassiana for insect control: current status” Biocontrol Science and Technology, 4, 3-34, 1994
  • Monzon A.J., Guharay F., Klingen I., “Natural occurrence of Beauveria bassiana in Hypothenemus hampei (Coleoptera: Curculionidae) populations in unsprayed coffee fields” Journal of Invertebrate Pathology, 97, 134-141, 2008
  • Chikwenhere G.P.., “Potential effects of Beauveria bassiana (Balsmo) Vuillemin on Neochetina bruchi Hustache (Coleoptera: Curculionidae), a biological control agent of water hyacinth” Biological Control, 21, 105-110, 2001
  • Gulsar Banu J., Subahasan K., Iyer R., “Occurrence and distribution of entomopathogenic nematodes in white grub endemic areas of Kerala” Journal of Plant Crops, 32, 333-334, 2004
  • Bidochka M.J., Menzies F.V., Kamp A.M., “Genetic groups of the insect-pathogenic fungus Beauveria bassiana are associated with habitat and thermal growth preferences” Archives of Microbiology, 178, 531-537, 2002
  • Maurer P., Couteaudier Y., Girard P.A., Bridge P.D., Riba G., “Genetic diversity of Beauveria bassiana and relatedness to host insect range” Mycological Research, 10, 159-164, 1997
  • Sevim A., Hofte M., Demirbag Z., “Genetic variability of Beauveria bassiana and Metarhizium anisopliae var. Anisopliae isolates obtained from the Eastern Black Sea Region of Turkey” Turkish Journal of Biology, 36, 255-265, 2012
  • The Ministry of Agriculture of Turkey, “Struggle with HazelnutPests and Diseases” Turkey, 1992
  • Tafoya F., Zuniga-Delgadillo M., Alatorre R., Cibrian-Tovar J., Stanley D., “Pathogenicity of Beauveria bassiana (Deuteromycota: Hyphomycetes) against the cactus weevil, Metamasius spinolae (Coleoptera: Curculionidae) under laboratory conditions” Florida Entomologist, 87(4), 533-536, 2013
  • Lo Verde G., Torta L., Mondello V., Caldarella C.G., Burruano S., Caleca V., “Pathogenicity bioassays of isolates of Beauveria bassiana on Rhynchophorus ferrugineus. Pest Management Science, doi: 10.1002/ps.3852, 2014
Toplam 42 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Mühendislik
Bölüm Biyoloji
Yazarlar

Ali Sevim Bu kişi benim

Elif Sevim Bu kişi benim

İsmail Demir

Zihni Demirbağ

Yayımlanma Tarihi 3 Şubat 2015
Yayımlandığı Sayı Yıl 2014 Cilt: 3 Sayı: 2

Kaynak Göster

APA Sevim, A., Sevim, E., Demir, İ., Demirbağ, Z. (2015). Molecular Characterization and Pathogenicity of Beauveria bassiana Isolated from Rhynchites bacchus L. (Coleoptera: Rhynchitidae). Nevşehir Bilim Ve Teknoloji Dergisi, 3(2), 33-47. https://doi.org/10.17100/nevbiltek.210929
AMA Sevim A, Sevim E, Demir İ, Demirbağ Z. Molecular Characterization and Pathogenicity of Beauveria bassiana Isolated from Rhynchites bacchus L. (Coleoptera: Rhynchitidae). Nevşehir Bilim ve Teknoloji Dergisi. Şubat 2015;3(2):33-47. doi:10.17100/nevbiltek.210929
Chicago Sevim, Ali, Elif Sevim, İsmail Demir, ve Zihni Demirbağ. “Molecular Characterization and Pathogenicity of Beauveria Bassiana Isolated from Rhynchites Bacchus L. (Coleoptera: Rhynchitidae)”. Nevşehir Bilim Ve Teknoloji Dergisi 3, sy. 2 (Şubat 2015): 33-47. https://doi.org/10.17100/nevbiltek.210929.
EndNote Sevim A, Sevim E, Demir İ, Demirbağ Z (01 Şubat 2015) Molecular Characterization and Pathogenicity of Beauveria bassiana Isolated from Rhynchites bacchus L. (Coleoptera: Rhynchitidae). Nevşehir Bilim ve Teknoloji Dergisi 3 2 33–47.
IEEE A. Sevim, E. Sevim, İ. Demir, ve Z. Demirbağ, “Molecular Characterization and Pathogenicity of Beauveria bassiana Isolated from Rhynchites bacchus L. (Coleoptera: Rhynchitidae)”, Nevşehir Bilim ve Teknoloji Dergisi, c. 3, sy. 2, ss. 33–47, 2015, doi: 10.17100/nevbiltek.210929.
ISNAD Sevim, Ali vd. “Molecular Characterization and Pathogenicity of Beauveria Bassiana Isolated from Rhynchites Bacchus L. (Coleoptera: Rhynchitidae)”. Nevşehir Bilim ve Teknoloji Dergisi 3/2 (Şubat 2015), 33-47. https://doi.org/10.17100/nevbiltek.210929.
JAMA Sevim A, Sevim E, Demir İ, Demirbağ Z. Molecular Characterization and Pathogenicity of Beauveria bassiana Isolated from Rhynchites bacchus L. (Coleoptera: Rhynchitidae). Nevşehir Bilim ve Teknoloji Dergisi. 2015;3:33–47.
MLA Sevim, Ali vd. “Molecular Characterization and Pathogenicity of Beauveria Bassiana Isolated from Rhynchites Bacchus L. (Coleoptera: Rhynchitidae)”. Nevşehir Bilim Ve Teknoloji Dergisi, c. 3, sy. 2, 2015, ss. 33-47, doi:10.17100/nevbiltek.210929.
Vancouver Sevim A, Sevim E, Demir İ, Demirbağ Z. Molecular Characterization and Pathogenicity of Beauveria bassiana Isolated from Rhynchites bacchus L. (Coleoptera: Rhynchitidae). Nevşehir Bilim ve Teknoloji Dergisi. 2015;3(2):33-47.

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