Araştırma Makalesi
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SDS-PAG Elektroforezinin Et ve Sakatat Analizinde Kullanılması

Yıl 2022, Sayı: 4, 25 - 34, 30.09.2022

Öz

Sodyum Dodesil sülfat poliakrilamid jel elektroforezi (SDS-PAGE), bir protein karışımının bileşenleri ayrı ayrı çözmek için en yaygın kullanılan analitik yöntemdir. Ayrıca bir proteinin saflığını elektroforetik bir yöntemle değerlendirmek neredeyse zorunlu hale gelmiştir. SDS-PAGE, proteinlerin moleküler boyuttaki farklılıkları ile jel matrisi boyunca %1' den daha az farklılık gösteren elektroforetik hareketliliklerini aynı anda kullanarak proteinleri birbirinden ayırır. Teknik aynı zamanda proteinlerin moleküler ağırlıklarını tahmin etme de güçlü bir araçtır.
Protein analizinde vazgeçilmez bir araç olarak SDS-PAGE'in başarısı, elektroforetik hareketliliğin bir proteinin moleküler kütlesi ile korelasyonuna izin vermektedir. Ve günümüzde başarılı bir SDS-PAGE analizi sadece ve sadece dikkatli numune hazırlama ve yüklemeden önce işlemlere bağlıdır.
Bu makalede en basit SDS-PAGE analizinin farklı et ve sakatat örneklerinin ayrımında kullanılabileceği anlatılmaktadır.

Kaynakça

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Toplam 26 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Analitik Biyokimya, Gıda Mühendisliği
Bölüm Araştırma Makaleleri
Yazarlar

Özgür Kaynar 0000-0002-2875-423X

İsmail Erim Köseoğlu 0000-0001-7924-8209

Erken Görünüm Tarihi 27 Eylül 2022
Yayımlanma Tarihi 30 Eylül 2022
Gönderilme Tarihi 21 Temmuz 2022
Yayımlandığı Sayı Yıl 2022 Sayı: 4

Kaynak Göster

APA Kaynar, Ö., & Köseoğlu, İ. E. (2022). SDS-PAG Elektroforezinin Et ve Sakatat Analizinde Kullanılması. Akademik Et Ve Süt Kurumu Dergisi(4), 25-34.